Composition for reducing the phytotoxicity of agricultural machine oil containing a sucrose fatty acid ester and/or urea

ABSTRACT

A method for reducing the phytotoxicity of agricultural machine oil in its application to an agricultural or horticultural plant to exterminate noxious insects and acarines, characterized in that, prior to or at the same time as the application of the machine oil, a sucrose fatty acid ester is applied to the agricultural or horticultural plant in an amount of from 0.1 to 50 parts by weight per 100 parts by weight of the machine oil, in the form of an aqueous solution containing from 0.001 to 1 wt/v % of the ester.

This is a continuation of application Ser. No. 564,188, filed Dec. 22, 1983, now U.S. Pat. No. 4,541,859.

The present invention relates to a method for reducing the phytotoxicity by agrochemicals, which frequently occurs when an agricultural machine oil is applied to plants alone or in combination with other agricultural chemicals by combined application or short interval application, and to an insecticidal and acaricidal composition useful for the method.

The agricultural machine oil is mainly used for the extermination of scale insects or acarines breeding on fruit trees such as citrus, apple, peach or pear trees. Such machine oil is regarded as an important agricultural chemical, since it does not create any substantial resistance, which insects or acarines may acquire when conventional synthetic insecticides or acaricides are employed, and it is inexpensive. On the other hand, it is known that when applied to plants, the agricultural machine oil presents certain adverse effects or phytotoxicity to the plants. This is a main factor which limits the usefulness of the machine oil. As such adverse effects caused by the application of the agricultural machine oil, there may be mentioned oil specks formed on the surface of fruits and leaves, falling off of the leaves or the quality degradation of the fruits such as a decrease of a sugar content in the fruits, an increase of the acidity or inferior coloration. At the same time, the photosynthetic ability of the plant leaves will be substantially lowered by the application of the agricultural machine oil, and it is reported that it takes a few months for the recovery of the photosynthetic ability. Further, when used in combination with other agricultural chemicals for combined application or short interval application, the agricultural machine oil frequently serves to promote the phytotoxicity of the agricultural chemicals or gives rise to a phytotoxicity which does not appear when the agricultural chemicals are used alone independently. Thus, the usefulness of the machine oil is thereby substantially restricted.

In order to solve the above-mentioned problems, it has been proposed to improve the quality of the agricultural machine oil by refining it to lower the viscosity. By such a quality improvement, the adverse effects can be reduced to some extent. However, the production cost increases as the process requires an additional refining step. Further, the reduction of the adverse effets is not necessarily adequate.

On the other hand, it has been proposed to use surfactants for the reduction of the phytotoxicity (Japanese Examined Patent Publications No. 14613/1978 and No. 40688/1981). However, these proposals are intended to reduce the phytotoxicity of copper sulfate or copper chloride to plants by dispersing the copper sulfate or copper chloride in machine oil in the presence of a nonionic surfactant.

The present invention has been accomplished as a result of extensive researches on various surfactants with an aim to reduce the phytotoxicity of the agricultural machine oil without impairing the insecticidal and acaricidal activities.

Namely, the present invention provides a method for reducing the phytotoxicity of agricultural machine oil, which is characterized in that a sucrose fatty acid ester is applied prior to or at the same time as the application of the agricutural machine oil. The present invention also provides a composition useful for the method. In a preferred embodiment of the present invention, urea is used in combination with the sucrose fatty acid ester.

Now, the present invention will be described in detail with reference to the preferred embodiments.

Various sucrose fatty acid esters are commercially available, in which the kind of the fatty acid or the degree of esterification varies. Any one of them may optionally be employed depending upon the particular purpose.

From the viewpoint of convenience for the preparation of a formulation by mixing it with the agricultural machine oil, the sucrose fatty acid ester should preferably have a low HLB. Whereas, from the viewpoint of the solubility and dispersibility in water for its application in a diluted form, it should preferably have a high HLB. The HLB may be at any level, when the sucrose fatty acid is used in combination with an emulsifier or a solvent for the preparation of a formulation or a diluted solution for application. However, the sucrose fatty acid ester preferably has a HLB of from 9 to 17. The fatty acid moiety thereof preferably has from 14 to 18 carbon atoms, and the monoester content in the sucrose fatty acid ester is preferably from 40 to 80%.

The agricultural machine oil is commercially available usually in the form of an emulsifiable concentration. At the time of the application, it is diluted with water and used in the form of an emulsion containing from 0.25 to 8 v/v % of the machine oil.

The sucrose fatty acid ester is usually applied in an amount of from 0.1 to 50 parts by weight, preferably from 0 5 to 20 parts by weight, per 100 parts by weight of the agricultural machine oil. The sucrose fatty acid ester of this amount is applied prior to or simultaneously with the application of the agricultural machine oil, in the form of an aqueous solution containing from 0.001 to 1 wt/v %, preferably from 0.01 to 0.2 wt/v %. In some cases, it may be used in the form of a mixture with the agricultural machine oil.

If the amount of the sucrose fatty acid ester is less than the above-mentioned range, the effectiveness for the reduction of the phytotoxicity tends to be small. On the other hand, if the amount exceeds the above- mentioned range, it will be necessary to increase the amount of the emulsifier or the solvent required for the preparation of the formulation.

When urea is to be employed in combination with the sucrose fatty acid ester, urea is usually used in an amount of from 1 to 300 parts by weight, preferably from 5 to 100 parts by weight, per 100 parts by weight of the agricultural machine oil, in the form of an aqueous solution containing from 0.01 to 5 wt/v % of the urea. If the amount of the urea is too small, no adequate effectiveness for the reduction of the phytotoxicity will be obtained. On the other hand, if the amount is excessive, the urea concentration at the time of the application will be unduly high, whereby it is likely to lead to an adverse effect to the plants.

In the present invention, the sucrose fatty acid ester may be used in combination with a conventional emulsifier, wettable powder, solvent, herbicide, fungicide, insecticide or fertilizer. Such an additive may be incorporated at the time of the application or during the preparation of the composition.

According to the present invention, an aqueous sucrose fatty acid ester is applied prior to the application of agricultural machine oil, or a composition comprising agricultural machine oil and a sucrose fatty acid ester is diluted with water and applied in the same manner as in the case of the application of usual agricultural machine oil, whereby it is possible to substantially reduce the phytotoxicity of the agricultural machine oil, such as formation of oil specks on the leaves and fruits, falling off of the leaves, degradation of the quality of the fruits such as a decrease of the sugar content, an increase of the acidity or inferior coloring of the fruits, or a decrease of photosynthetic ability of the leaves, which used to be caused by the application of agricultural machine oil. Further, it is thereby possible to reduce a phytotoxicity which is caused by combined application or short interval application of agricultural machine oil together with other agricultural chemicals such as 2,3-dithiano-1,4-dithianoneanthraquinone. The mechanism for the reduction of the phytotoxicity according to the process of the present invention is not clearly understood. It is likely, however, that the sucrose fatty acid ester serves to prevent the local concentration of agricultural chemicals such as agricultural machine oil resulting from the penetration and transfer of the agricultural chemicals in the leaves or the fruit surfaces, and to promote the proper function of various metabolisms in the plant body, and urea provides a nutrient as a nitrogen source and thus serves for the encouragement of the plants, whereby not only the emergence of the phytotoxicity is prevented but also the resistibility of the plants against the phytotoxicity is improved.

Now, the present invention will be described in further detail with reference to Examples. However, it should be understood that the present invention is by no means restricted by these specific Examples.

In the following Examples, Spindlon emulsifiable concentration manufactured by Mikasa Chemical Industry Co., Ltd. was used as the machine oil emulsifiable concentration, and Ryoto Sugar Esters (manufactured by Ryoto Co., Ltd.) as identified in the following Table 1 were used as the sucrose fatty acid esters.

                  TABLE 1                                                          ______________________________________                                         Trade-                   Ester compo-                                          names                    sition (%)                                            Cane  Ryoto      Bound fatty acids (%) Di-,                                    sugar Sugar          Stearic                                                                              Palmit-                                                                              Oleic Mono- tri-                              esters                                                                               Esters  HLB    acid  ic acid                                                                              acid  ester ester                             ______________________________________                                         SE-1  S-1570  15     70    30    --    70    30                                SE-2  P-1570  15     30    70    --    70    30                                SE-3  O-1570  15     --    --    70    70    30                                SE-4  O- 570   5     --    --    70    35    65                                SE-5  S- 570   5     70    30          35    65                                SE-6  L-1540  15     Laulic acid 50                                                                               70    30                                    ______________________________________                                    

EXAMPLES 1 to 6 and COMPARATIVE EXAMPLE

The machine oil emulsifiable concentration and SE-3 were diluted with water to bring the machine oil concentration to 1% (v/v) and the SE-3 concentration to those identified in Table 2, and they were dissolved to obtain emulsions for application. Each emulsion was applied to a potted three year old plant of Satsuma mandarine orange (Citrus unshiu Marc) so that the leaves were sufficiently wetted. Three days later, the formation of oil specks as a characteristic phytotoxicity of the machine oil emulsion was evaluated with respect to new leaves developed this year, in accordance with the evaluation standards as identified in Table 3.

The results thereby obtained are shown in Table 2. It is evident from Table 2 that in the case where the machine oil emulsifiable concentration was used alone, oil specks were formed on the leaf surface, whereas by the presence of the sucrose fatty acid ester, the formation of the oil specks was remarkable suppressed.

                  TABLE 2                                                          ______________________________________                                                     Concentration of                                                                         Degree of                                                            SE-3 (%, w/v)                                                                            phytotoxicity                                            ______________________________________                                         Comparative   0           +++                                                  Example 1                                                                      Example 1     0.0025      ++                                                   Example 2     0.005       +                                                    Example 3     0.01        +                                                    Example 4     0.04        +                                                    Example 5     0.08        +                                                    Example 6     0.2         ±                                                 ______________________________________                                    

                  TABLE 3                                                          ______________________________________                                         Degree of                                                                      phytotoxicity Evaluation standards                                             ______________________________________                                         +++           Oil specks formed on at least                                                  30% of the entire surface area                                                 of the leaf                                                      ++            Oil specks formed on 10-30%                                                    of the entire surface area of                                                  the leaf                                                         +             Oil specks formed on less than                                                 10% of the entire surface area                                                 of the leaf                                                      ±          Oil specks slightly observed                                     ______________________________________                                    

EXAMPLES 7 to 9

The machine oil emulsifiable concentration and various sucrose fatty acid esters as identified in Table 4 were diluted with water to bring their concentrations to 1% (v/v) and 0.01% (w/v), respectively, and they were dissolved to obtain emulsions for application. In the same manner as in Example 1, the application treatment was conducted, and the formation of the phytotoxicity was evaluated.

The results thereby obtained are shown in Table 4. If is evident from Table 4 that each kind of the sucrose fatty acid esters was effective in the reduction of the phytotoxicity.

                  TABLE 4                                                          ______________________________________                                                               Degree of                                                           Cane sugar esters                                                                         phytotoxicity                                            ______________________________________                                         Comparative  --           +++                                                  Example 1                                                                      Example 7    SE-1         +                                                    Example 8    SE-2         +                                                    Example 9    SE-4         +                                                    ______________________________________                                    

EXAMPLES 10 and 11

The machine oil emulsifiable concentration and SE-3 were diluted with water to bring their concentrations to 1% (v/v) and 0.08% (w/v), respectively, and they were dissolved to obtain an emulsion for application. Another emulsion was prepared in the same manner except that 1% (w/v) of urea was added. In the same manner as in Example 1, these emulsions were applied, and the formation of the phytotoxicity was evaluated.

The results thereby obtained are shown in Table 5. It is evident from Table 5 that the phytotoxicity can further be reduced by the combination of the sucrose fatty acid ester with urea.

                  TABLE 5                                                          ______________________________________                                                Concentration                                                                            Concentration                                                        of SE-3   of urea      Degree of                                               (%, w/v)  (%, w/v)     phytotoxicity                                    ______________________________________                                         Comparative                                                                             0           0            +++                                          Example 1                                                                      Example 10                                                                              0.08        0            +                                            Example 11                                                                              0.08        1            ±                                         ______________________________________                                    

EXAMPLES 12 to 14 and COMPARATIVE EXAMPLE 2

The machine oil emulsifiable concentration, SE-3 and urea were diluted with water to bring their concentrations to the respective values as identified in Table 6, and they were dissolved to obtain emulsions for application. Each emulsion was applied to a potted three years old plant of Satsuma mandarine orange so that the leaves were adequately wetted. The photosynthesis rates were measured prior to the application, one day after the application and four days after the application. The results thereby obtained are shown in Table 6, in which the photosynthesis rates are represented by the relative values based on the photosynthesis rate prior to the application being taken as 100.

The measurement of the photosynthesis rates was conducted by placing the sample plants in a closed phytotron (30°C.) and measuring the change of the carbondioxide concentration in the phytotron by means of an infrared gas analyzer. The apparent photosynthesis rate was obtained from the reducing rate of the carbondioxide concentration under illumination, and the respiration rate was obtained from the increasing rate of the carbondioxide concentration under a blacked out condition. The sum of the apparent photosynthesis rate and the respiration rate was taken as the true photosynthesis rate. From the results of the measurement, it was found that by the application of the machine oil emulsion, the photosynthesis rate was remarkably reduced, whereas when the sucrose fatty acid ester was used in combination, the reduction of the photosynthesis rate is substantially moderated, and when urea was also used in combination, the reduction of the photosynthesis rate is further minimized.

                  TABLE 6                                                          ______________________________________                                         Compositions of the                                                            emulsions          Photosynthesis rates                                        Machine                (Relative values)                                       oil emul-                                4 days                                sifiable                   Prior to                                                                              One day                                                                               after                                 concen-      SE-3   Urea   the ap-                                                                               after the                                                                             the ap-                               tration      (%,    (%,    plica- applica-                                                                              plica-                                (%, v/v)     v/v)   v/v)   tion   tion   tion                                  ______________________________________                                         Compara-                                                                               1        0      0    100    78     83                                  tive                                                                           Example 2                                                                      Example 1        0.01   0    100    86     87                                  12                                                                             Example 1        0.2    0    100    89     93                                  13                                                                             Example 1        0.2    1    100    94     97                                  14                                                                             ______________________________________                                    

EXAMPLES 15 to 22 and COMPARATIVE EXAMPLES 3 to 15

By using the machine oil emulsifiable concentration, SE-3, urea, an insecticide (dimethoate emulsifiable concentration manufactured by Tomono Nohyaku Co., Ltd.) and a fungicide (Spatcide wettable powder manufactured by Kumiai Chemical Industry Co., Ltd.), comparative tests (Test Nos. 1-5) as shown in Table 7 were set up each for comparison of a combined application with a control. Test No. 6 is a blank test where no agricultural chemical was applied.

For each comparative test, three Satsuma mandarine orange trees of six years old were used. In each tree, a half thereof was used for testing the combined application and the other half was used for testing the control.

The concentrations of the chemicals in the emulsions for application were as follows: 1% (v/v) of the machine oil emulsifiable concentration, 0.08% (w/v) of SE-3, 1% (w/v) of urea, 0.1% (v/v) of the insecticide and 0.13% (w/v) of the fungicide.

In each comparative test, the application was conducted twice, i.e. the second application was made on the 37th day after the first application. Each time, the emulsions were applied by means of a sprayer so that the test tree leaves were adequately wetted (i.e. 2-2.3 liters per tree). After the second application, the phytotoxicities to new leaves, old leaves and fruits were evaluated by examination of the rate of oil-specked leaves or fruits in all leaves or fruits, and the number of fallen leaves was also investigated. The results thereby obtained are shown in Table 7.

Further, on the 77th day after the second application, ten fruits were taken from each half of each tree, and their weights, the sugar content and the citric acid content of the fruit juice were measured, and the coloring, the uniformity of the coloring and the stains of the fruit skin were examined. The results thereby obtained are shown in Table 8. The sugar content was measured by Brix sugar meter, and the citric acid content was obtained by calculating the amount of citric acid from the titer obtained by titration with a sodium hydroxide standard solution.

As shown in Table 7, the phytotoxicities to the leaves and fruits or the increase of the number of fallen leaves caused by the machine oil emulsifiable concentration or the combined use of the machine oil emulsifiable concentration with other agricultural chemicals, can be reduced to some extent by a combined use of urea. However, when the sucrose fatty acid ester or a combination of the sucrose fatty acid ester with urea was employed, the effectiveness for the reduction of the phytotoxicities becomes more distinct. On the other hand, with respect to the quality of the fruits, the combined use of urea gives certain effectiveness to prevent the decrease of the sugar content and the increase of citric acid, whereas when the sucrose fatty acid ester or a combination of the sucrose fatty acid ester with urea was employed, not only such effectiveness but also other effectiveness such as an increase of the weight of the fruits, good coloring and little stains, are obtainable.

                                      TABLE 7                                      __________________________________________________________________________                                      Phytotoxicities                                                                                        Number of                                                                      fallen                                                 Rate of Rate of Rate of leaves                                                 oil-specked                                                                            oil-specked                                                                            oil-specked                                                                            (Number/                                               new leaves (%)                                                                         old leaves (%)                                                                         fruits                                                                                 tree)                        Test                      Number of days after the second                                                application                                          Nos.                                                                              Agricultural chemicals 7 days                                                                             33 days                                                                            7 days                                                                             33 days                                                                            7 days                                                                             33                                                                                 7                     __________________________________________________________________________                                                              days                  Comparative                                                                           1  Machine oil emulsifiable concentration                                                                88  75  93  50  98  42  4                     Example 3                                                                      Example 15                                                                               Machine oil emulsifiable concentration + SE-3                                                         72  53  43  25  60   7  1                     Comparative                                                                           2  Machine oil emulsifiable concentration                                                                88  92  100 80  95  65  5                     Example 4                                                                      Comparative                                                                              Machine oil emulsifiable concentration + Urea                                                         85  55  50  35  47  25  1                     Example 5                                                                      Comparative                                                                           3  Machine oil emulsifiable concentration                                                                100 72  92  43  47  40  4                     Example 6                                                                      Example 16                                                                               Machine oil emulsifiable concentration +                                                              75  25  48  18  15   3  1                               SE-3 + Urea                                                          Comparative                                                                           4  Machine oil emulsifiable concentration +                                                              100 82  92  78  85  62  3                     Example 7 Insecticide                                                          Example 17                                                                               Machine oil emulsifiable concentration +                                                              53  35  57  23  40  36  1                               Insecticide + SE-3                                                   Comparative                                                                           5  Machine oil emulsifiable concentration +                                                              67  37  80  27  48  48  4                     Example 8 Bactericide                                                          Example 18                                                                               Machine oil emulsifiable concentration +                                                              32  35  35  18  34  13  1                               Bactericide + SE-3                                                   Comparative                                                                           6  None                    0   0   0   0   0   0  0                     Example 9                                                                      __________________________________________________________________________

                                      TABLE 8                                      __________________________________________________________________________                                                    Sugar-acid                                                                           Coloring and fruit                                         Weight of Citric                                                                             ratio skin condition (as               Test                      the fruit                                                                            Sugar                                                                              acid                                                                               (Sugar/                                                                              compared with the                Nos.                                                                              Agricultural chemicals (g)   (%) (%) citric acid)                                                                         respective                __________________________________________________________________________                                                          control)                  Comparative                                                                           1  Machine oil emulsifiable concentration                                                                113   7.0 1.3 5.4   (Control)                 Example 10                                                                     Example 19                                                                               Machine oil emulsifiable concentration + SE-3                                                         133   7.1 1.1 6.5   Coloring-good                                                                  Stains on the                                                                  skin-little               Comparative                                                                           2  Machine oil emulsifiable concentration                                                                142   7.0 1.3 5.4   (Control)                 Example 11                                                                     Comparative                                                                              Machine oil emulsifiable concentration + Urea                                                         137   7.2 1.2 6.0   Stains on the skin        Example 12                                           substantial               Comparative                                                                           3  Machine oil emulsifiable concentration                                                                121   6.9 1.4 4.9   (Control)                 Example 13                                                                     Example 20                                                                               Machine oil emulsifiable concentration +                                                              124   7.0 1.3 5.4   Coloring good and                   SE-3 + Urea                                uniform Stains on                                                              the skin-little           Comparative                                                                           4  Machine oil emulsifiable concentration +                                                              106   6.9 1.5 4.6   (Control)                 Example 14                                                                               Insecticide                                                          Example 21                                                                               Machine oil emulsifiable concentration +                                                              117   7.0 1.4 5.0   Stains on the                       Insecticide + SE-3                         skin-little               Comparative                                                                           5  Machine oil emulsifiable concentration +                                                              110   6.7 1.5 4.5   (Control)                 Example 15                                                                               Bactericide                                                          Example 22                                                                               Machine oil emulsifiable concentration +                                                              123   6.8 1.4 4.9   Coloring-good                       Bactericide + SE-3                                                   __________________________________________________________________________

EXAMPLES 23 to 26 and COMPARATIVE EXAMPLES 16 to 19

The effectiveness for the reduction of the phytotoxicities to Satsuma mandarine orange trees of six years old was compared with respect to the combined application or the short interval application of the machine oil emulsifiable concentration and a fungicide (Delan wettable powder containing 2,3-dithiano-1,4-dithianoneanthraquinone as the active ingredient, manufactured by Mikasa Chemical Industry Co., Ltd.).

For the combined application, emulsions for combined application as shown in Table 9 were prepared by using the machine oil emulsifiable concentration, the fungicide, urea and SE-3. Three orange trees were used for each of the four tests. The rate of formation of brown specks which started to form about one week after the application of each emulsion, was measured and presented in Table 9 as the phytotoxicity rate.

In the short interval application tests, the fungicide was firstly applied to all orange trees, as shown in Table 10. Twelve days after the first application, each of the four emulsions for the second application as identified in Table 10 was applied to three orange trees, respectively. Then, the phytotoxicity rate was determined in the same manner as mentioned above. The results thereby obtained are shown in Table 10.

From the results, it is evident that with respect to the effectiveness for the reduction of phytotoxicity in the combined and short interval applications, the combined use of urea is effective to some extent, whereas the combined use of the sucrose fatty acid ester presents remarkable effectiveness, and the combination of the sucrose fatty acid ester with urea further improves the effectiveness.

                  TABLE 9                                                          ______________________________________                                         Combined application                                                           Machine                                                                        oil emul-                                                                      sifiable                                                                       concen-      Bacteri-          Urea Phytotoxicity                              tration      cide     SE-3     (%,  rate                                       (%, v/v)     (%, w/v) (%, w/v) w/v) (%)                                        ______________________________________                                         Compara-                                                                               1.0      0.2      --     --   100                                      tive                                                                           Example                                                                        16                                                                             Example 1.0      0.2      0.08   --   40                                       23                                                                             Compara-                                                                               1.0      0.2      --     1.0  60                                       tive                                                                           Example                                                                        17                                                                             Example 1.0      0.2      0.08   1.0  10                                       24                                                                             ______________________________________                                    

                  TABLE 10                                                         ______________________________________                                         First                                                                          applica-     Second application                                                tion         Machine oil                                                       Bacteri-     emulsifiable                                                                              SE-3   Urea Phytotoxicity                              cide         concentra- (%,    (%,  rate                                       (%, w/v)     tion (%, v/v)                                                                             w/v)   w/v) (%)                                        ______________________________________                                         Compara-                                                                               0.2      1.0        --   --   100                                      tive                                                                           Example                                                                        18                                                                             Example 0.2      1.0        0.08 --    8                                       25                                                                             Compara-                                                                               0.2      1.0        --   1.0   70                                      tive                                                                           Example                                                                        19                                                                             Example 0.2      1.0        0.08 1.0   5                                       26                                                                             ______________________________________                                    

EXAMPLES 27 to 30 and COMPARATIVE EXAMPLES 20 to 26

The emulsions having various compositions as shown in Table 11 (as the fungicide, the same fungicide as used in Examples 23 to 26 was employed) were applied to the foliage of Chinese cabbages having a leaf age of 3.5 (11 days after seeding) in an application rate of 100 liter/10 a. Three days later, the phytotoxicity appeared on the leaf surface was examined and classified into five levels (i.e. index numbers 0 to 5 where 0 indicates that no phytotoxicity appeared, and 5 indicates that the phytotoxicity appeared on the entire leaf surface). The phytotoxicity degree was calculated from the results of the evaluation in accordance with the following formula: ##EQU1##

The results thereby obtained are shown in Table 11. From the results, it is evident that the phytotoxicity caused by the single use of the machine oil emulsifiable concentration or the phytotoxicity caused by the combined use of the machine oil emulsifiable concentration with the fungicide, can be reduced to some extent by the presence of urea, whereas the effectiveness for the reduction of the phytotoxicity is more distinct in the case where the sucrose fatty acid ester was present, and the effectiveness is further improved by the combined use of the sucrose fatty acid ester with urea.

                  TABLE 11                                                         ______________________________________                                         Composition of emulsions for                                                   application                                                                    Machine oil                                                                    emulsifiable   Bacteri- SE-3   Urea Phytotoxicity                              concentra-     cide     (%,    (%,  rate                                       tion (%, v/v)  (%, w/v) w/v)   w/v) (%)                                        ______________________________________                                         Compara-                                                                               1.0        --       --   --   2.3                                      tive                                                                           Example                                                                        20                                                                             Compara-                                                                               --         --       0.2  --   0.0                                      tive                                                                           Example                                                                        21                                                                             Example 1.0        --       0.2  --   0.0                                      27                                                                             Compara-                                                                               1.0        --       --   1.0  1.7                                      tive                                                                           Example                                                                        22                                                                             Example 1.0        --       0.2  1.0  0.0                                      28                                                                             Compara-                                                                               --         1.0      --   --   0.0                                      tive                                                                           Example                                                                        23                                                                             Compara-                                                                               1.0        1.0      --   --   67                                       tive                                                                           Example                                                                        24                                                                             Example 1.0        1.0      0.2  --   49                                       29                                                                             Compara-                                                                               1.0        1.0      --   1.0  62                                       tive                                                                           Example                                                                        25                                                                             Example 1.0        1.0      0.2  1.0  40                                       30                                                                             Compara-                                                                               No treatment          0.0                                              tive                                                                           Example                                                                        26                                                                             ______________________________________                                    

EXAMPLES 31 to 33 and COMPARATIVE EXAMPLES 27 and 28

The effectiveness for the reduction of the phytotoxicity to Satsuma mandarine orange trees of 16 years old was tested with respect to short interval application of the sucrose fatty acid ester and the machine oil emulsifiable concentration.

The solutions for the first application as shown in Table 12 were prepared by using SE-3, urea and a fungicide (the same fungicide as used in Examples 23 to 26) For each test, one branch of the orange tree was used. The five solusions for the first application were applied to the respective five branches. Five days later, the emulsion of the machine oil emulsifiable concentrate was prepared as identified in Table 12 and applied to each branch as the second application. On the 8th day after the second application, the rate of formation of oil specks on the leaf surface was examined. The rate of formation of oil specks is presented in Table 12 as the phytotoxicity rate.

From the results, shown in Table 12, it is evident that when the sucrose fatty acid ester is applied prior to the application of the machine oil, the effectiveness for the reduciton of the phytotoxicity is obtainable, when urea and the sucrose fatty acid ester are used in combination, this effectiveness is further increased. Further, it has been found that the sucrose fatty acid ester is effective to suppress the phytotoxicity rate which was increased by the short interval application of the fungicide and the machine oil emulsifiable concentration.

                  TABLE 12                                                         ______________________________________                                                            Second ap-                                                                     plication                                                           First application                                                                         Machine oil                                                 SE-3       Urea   Bacteri- emulsifiable                                                                            Phytotoxicity                              (%,        (%,    cide     concentra-                                                                              rate                                       w/v)       w/v)   (%, w/v) tion (%, v/v)                                                                           (%)                                        ______________________________________                                         Compara-                                                                               --     --     --     0.7      97                                       tive                                                                           Example                                                                        27                                                                             Example 0.08   --     --     0.7      67                                       31                                                                             Example 0.08   0.4    --     0.7      63                                       32                                                                             Compara-                                                                               --     --     0.2    0.7      100                                      tive                                                                           Example                                                                        28                                                                             Example 0.08   --     0.2    0.7      87                                       33                                                                             ______________________________________                                    

EXAMPLES 34 and 35 and COMPARATIVE EXAMPLES 29 to 36

The machine oil emulsifiable concentration and various surfactants as identified in Table 13 were diluted with water to bring their concentrations to those identified in Table 13, and they were dissolved to obtain emulsions for application. For each test, two stamps of cucumber of about 6-7 leaf stage were used. On the 7th day after the application of the emulsions, the degree of the phytotoxicity appeared on the leaves and the photosynthesis rate were examined. The degree of the phytotoxicity was evaluated in accordance with the evaluation standards of Table 14 with respect to the 4th to 8th leaves, i.e. a total of 8 leaves. The photosynthesis rate was measured in the same manner as in Examples 12 to 14 by using a closed micro chamber in stead of the phytotoron with respect to the 4th leaf of one of the two stamps of each test.

The results are shown in Table 13. From the results, it is evident that the sucrose fatty acid ester is superior to other surfactants in the effectiveness for the reduction of the phytotoxicity, and such effectiveness is particularly remarkable with respect to the photosynthesis rate.

                  TABLE 13                                                         ______________________________________                                         Agricultural chemicals                                                         Machine oil                        Photo-                                      emulsifiable                       synthesis                                   concen-                   Degree   rate                                        tration      Surfactant   of phyto-                                                                               (mg CO.sub.2                                (%, v/v)     (%, v/v)     toxicity dm.sup.-2 hr.sup.-1)                        ______________________________________                                         Compar-                                                                               --         --          -      22.6                                      ative                                                                          Example                                                                        29                                                                             Compar-                                                                               1          --          ++     16.0                                      ative                                                                          Example                                                                        30                                                                             Example                                                                               1         SE-3 (0.1)   +      21.4                                      34                                                                             Example                                                                               1         SE-3 (0.01)  ++     19.4                                      35                                                                             Compar-                                                                               1         Polyoxyethylene                                                                             +++    16.8                                      ative            sorbitane mono-                                               Example          oleate (0.1)                                                  31                                                                             Compar-                                                                               1         Polyoxyethylene                                                                             +++    17.9                                      ative            sorbitane mono-                                               Example          oleate (0.01)                                                 32                                                                             Compar-                                                                               1         Polyethylene +      17.6                                      ative            glycol mono-                                                  Example          stearate (0.1)                                                33                                                                             Compar-                                                                               1         Polyethylene +++    15.0                                      ative            glycol mono-                                                  Example          stearate (0.01)                                               34                                                                             Compar-                                                                               1         Polyoxyethylene                                                                             +++    17.3                                      ative            oleyl ether (0.1)                                             Example                                                                        35                                                                             Compar-                                                                               1         Polyoxyethylene                                                                             +++    14.5                                      ative            oleyl ether (0.01)                                            Example                                                                        36                                                                             ______________________________________                                    

                  TABLE 14                                                         ______________________________________                                         Degree of                                                                      phytotoxicity                                                                              Evaluation standard                                                ______________________________________                                         +++         Oil specks and color change-substantial                            ++          Oil specks and color change-distinctly                                         observed                                                           +           Oil specks-slightly observed                                       ______________________________________                                    

EXAMPLES 36 to 40 and COMPARATIVE EXAMPLE 37

The acaricidal effects of the compositions comprising the machine oil emulsifiable concentration and various sucrose fatty acid esters as identified in Table 15 were tested by a leaf disc method in a constant temperature room at 25°C. Water was put in an ice cream cup, and a filter paper with cut lines was placed in the ice cream cup so that the cut line portions were in contact with water. Another filter paper was placed thereon, and two leaf discs prepared by cutting a Satsuma mandarine orange leaf to form a disc having a diameter of 12 mm, were placed in each cup. On each disc, about 10 acarines were placed. Next day, after confirming the number of acarines settled on the discs, the emulsions containing 0.028% (w/v) of various sucrose fatty acid esters as identified in Table 15 and 0.35% (v/v) of the machine oil emulsifiable concentrate, were applied to the respective cups at a application rate of 50 mg/cm². Next day, the number of dead acarines on each leaf and the number of escaped acarines were counted, and the acaricidal rate was calculated in accordance with the following formula: ##EQU2##

The results thereby obtained are shown in Table 15. From the results, it is evident that all of the compositions of the present invention have remarkable acaricidal effects, although the acaricidal rates vary depending upon the type of the sucrose fatty acid ester used.

                  TABLE 15                                                         ______________________________________                                                   Cane sugar esters                                                                         Acaricidal rates (%)                                      ______________________________________                                         Example 36  SE-1         86.5                                                  Example 37  SE-2         94.6                                                  Example 38  SE-3         94.0                                                  Example 39  SE-5         53.4                                                  Example 40  SE-6         97.6                                                  Comparative --           46.7                                                  Example 37                                                                     ______________________________________                                     

We claim:
 1. An emulsifiable insecticidal and acaricidal concentrate, which comprises:100 parts by weight of an agricultural machine oil and from 0.1 to 50 parts by weight of a sucrose fatty acid ester.
 2. The composition according to claim 1, which contains from 0.5 to 20 parts by weight of the sucrose fatty acid ester per 100 parts by weight of the agricultural machine oil.
 3. The compostion according to claim 1, which comprises 100 parts by weight of the agricultural machine oil, from 0.1 to 50 parts by weight of the sucrose fatty acid ester and from 1 to 300 parts by weight of urea.
 4. The composition according to claim 3, which contains from 5 to 100 parts by weight of urea per 100 parts by weight of the agricultural machine oil.
 5. The composition according to claim 1, wherein the sucrose fatty acid ester has a HLB of from 9 to
 17. 6. The composition according to claim 5, wherein the fatty acid moiety of the sucrose fatty acid ester has from 14 to 18 carbon atoms, and the monoester content in the ester is from 40 to 80%.
 7. An emulsifiable insecticidal and acaricidal concentrate, which comprises: 100 parts by weight of an agricultural machine oil, from 0.1 to 50 parts by weight of a sucrose fatty acid ester and from 1 to 300 parts by weight of urea. 